Kit components - Core Kit

Reagent

Buffer 1

Wash Buffer

Buffer 3

miR-cel-39 synth. spike-in

Spike-in Dilution Mix

Protease Solution

Buffer 2

probe - cel-39

Circularization Reaction Mix

RT Reaction Mix

RT Primer (cel-39)

Rnase Free Water

PCR Primer mix (cel-39)

Storage

20°C to 25°C

4°C

4°C

-20°C

-20°C 

-20°C *

-20°C 

-20°C 

-20°C 

-20°C 

-20°C 

-20°C

-20°C 

* Keep at -20°C upon receipt. Store at 4°C after thawing for first use.

Tube

Buffer 1

Wash Buffer

Buffer 3

spike-in

Spike-in Dilution Mix

Protease Solution

Buffer 2

probe - cel-39

Circularization Reaction Mix

RT Reaction Mix

RT Primer (cel-39)

Water

PCR Primer mix (cel-39)

Kit components - miRNA-specific assays

User-supplied Reagents 

Streptavidin Magnetic beads (New England BioLabs, cat# S1420S)

CircLigase II (Epicentre, cat# CL9021K)

RNAseOUT (Thermo Fisher Scientific, cat# 10777019)

SuperScript II Reverse Transcriptase (ThermoFisher Scientific, cat#18064022) 

SYBR green reagents such as 2X iQ SYBR Green Supermix (BioRad, cat# 170-8880)

Rox reference dye (Thermo Fisher Scientific, cat# 12223012)